Figure 2
(a) Microscopic view of the cells from twin vials which were recovered from cryopreservation using two different methods: at 4 °C (top left) and at 37 °C in presence of Colcemid (top right), (b) quantitaive data of cell recovery with the two methods. (Error bar represents Mean ± SD from three independent experiments), (c) Damaged and live cell nucleus staining using Hoechst 33342 and PI. PI positive red/pink nuclear content represent dead cells or cell with compromised membrane integrity and blue nuclear content suggest live intact cell. Plasma membrane was marked using WGA staining visible in live intact cells. (d) Quantitative data of PI positive cells among the recovered cryopreserved cells using the two recovery methods (Error bar represents Mean ± SD from three independent experiments). (e) Progression of recovered mitotic cells from metaphase in presence and absence of Colcemid. (f) Quantitative data of kinetics of mitotic index in absence and presence of Colcemid after recovery from cryopreservation (Error bar represents Mean ± 1.96SE).
