Figure 3
Evaluation checkpoints of cryopreservation mitotic cells for PCC (a) Viability; Mitotic cells after differential staining of live dead cells (b) Mitotic index; Chromosomal spreads from mitotic cells in a microscopic view. Each chromosome spread represents one mitotic cell (encircled) and nuclei which did not form spread counted as non-mitotic cells. (c) Fusion/PCC index Chromosomal spread preparation on slide after cell fusion induced PCC. PCC spreads can be identified by presence of both metaphase chromosomes from mitotic cell and prematurely condensed chromosomes from lymphocytes (encircled). (d) Viability, mitotic index and PCC index of mitotic cells after variable duration of cryopreservation. (Error bar represents 1.96xSE of Mean).
