Figure 4
(a) A typical field of chromosome spread preparation G0-PCC FISH slide showing efficient fusion yield. PCC spread can be identified by the presence of painted human chromosome pairs 1, 2 and 4 in red green and yellow respectively (encircled), (b) G0-PCC spreads from one, two, three or four lymphocyte fusion to mitotic cell/s. Increase in number of lymphocyte chromosomes and size of the spread can be observed (top row, left to right). Multichannel view of spread shows red green and yellow fluorescent signals from painted chromosomes 1, 2 and 4, respectively. Since one lymphocyte caries one pair of each chromosome, presence of one, two, three and four pairs of each of the painted chromosomes confirm fusion of one two three and four lymphocyte respectively (left to right bottom row).
