Figure 3

Scheme of parabiosis experiments conducted using Rosa26^ECFP/+ and Rosa26^EGFP/+ mice. (a) Rosa26^ECFP/+ mice were paired with Rosa26^EGFP/+ mice and injected intraperitoneally with polyacrylamide beads 4 weeks after surgery. “EGFP” and “ECFP” denote a Rosa26^EGFP/+ and Rosa26^ECFP/+ mice, respectively. (b) Representative confocal image of the omenta of parabiotic Rosa26^ECFP/+ mice paired with Rosa26^EGFP/+ mice. EGFP⁺ cells were derived from Rosa26^EGFP/+ mice. (c) Representative flow cytometric plots of omental cells derived from Rosa26^ECFP/+ mice paired with Rosa26^EGFP/+ mice. Then, 7-AAD⁻/Lin⁻/ECFP⁻/EGFP⁺ cells (gate “H-Q4”) and 7-AAD⁻/Lin⁺/ECFP⁻/EGFP⁺ cells (gate “J-Q4”) were sorted for conducting scRNA-seq. Scale bar = 50 µm.