Figure 5

Representative confocal images of the omenta of parabiotic Rosa26^ECFP/+ mice paired with Rosa26^EGFP/+ mice. Rosa26^ECFP/+ mice were paired with Rosa26^EGFP/+ mice and injected with polyacrylamide beads 4 weeks after surgery. Mice were sacrificed 7 days after injection. Images (a–f′) show the omentum of the Rosa26^ECFP/+ mouse. (a) Hoechst nuclear staining. (b) Expression pattern of EGFP in the same field as (a). (c) Expression pattern of ECFP in the same field as (a). (d) Expression pattern of CD45 in the same field as (a). (e) Expression pattern of vimentin in the same field as (a). (f) Merged image of (a–e). (a′–f′) shows enlarged images of dotted line squares in (a–f). Purple arrowheads indicate CD45⁻/Vimentin⁺ cells expressing EGFP. Orange arrowhead indicates CD45⁺/Vimentin⁺ cells expressing EGFP. Scale bars = 50 µm.