Table 1 Binding to HER3-expressing tumor cells of scDb and scDb-scFv. EC50 [pM], Mean ± SD, n = 3. # For the HER3 low-expressing cell lines SW-620 and HCT116 the extrapolated values under the detection limit of 1900 molecules/cell (Qifikit) are depicted. *p < 0.05; **p < 0.01; n.s. p > 0.05; n.d., not determined.

From: A scDb-based trivalent bispecific antibody for T-cell-mediated killing of HER3-expressing cancer cells

Cell line

HER3 expression

scDb-scFv

scDb

p value

LIM1215

20,000 HER3/cell

200 ± 100

11,700 ± 6000

*

MCF-7

18,000 HER3/cell

30 ± 20

4700 ± 6200

*

BT-474

11,000 HER3/cell

200 ± 170

9700 ± 2200

**

FaDu

3000 HER3/cell

100 ± 30

–

n.d.

SW-620#

1160 HER3/cell

30 ± 3

30,800 ± 30,200

**

HCT116#

1520 HER3/cell

50 ± 10

–

n.d.

HT1080

HER3−

–

–

n.d.

MDA-MB-231

HER3−

–

–

n.d.

WM1791c

HER3−

–

–

n.d.

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