Figure 4

(a) Scores plot for principal component analysis comparing metabolites among diabetic streptozotocin (STZ)- or non-diabetic vehicle-treated C57BL/6 mice and diabetic and non-diabetic Swiss Webster mice. (b) Top 15 VIP scores that distinguish diabetic vs non-diabetic Swiss Webster mice. Heat map to right represents relative concentration of each metabolite. Two phosphatidylcholines, (c) PC ae C38:2 and (d) PC aa C42:5, showed the highest correlation to blood glucose in Swiss Webster mice tested by Pearson correlation, with p < 0.0001 for both. (e) Top 15 VIP scores that distinguish STZ- and vehicle-treated C57BL/6 mice with the concentrations of the top 3 scores plotted in (f). ***Significantly reduced in STZ vs vehicle, p < 0.0001. LF low-fat diet; HFL high-fat lard-based diet; HFO high-fat olive oil/fish oil-based diet. Early overnutrition LF: n = 6, 3 litters; early overnutrition HFL: n = 5, 3 litters; early overnutrition HFO: n = 6, 3 litters; control LF: n = 5, 3 litters; control HFL: n = 6, 3litters; control HFO: n = 6, 6 litters. STZ and vehicle, n = 4.