Table 4 Tissue weights and measures in fasted postnatal day 70 mice.

From: Early overnutrition in male mice negates metabolic benefits of a diet high in monounsaturated and omega-3 fats

Tissue/measure

Control LF

Control HFL

Control HFO

Early Over-nutrition LF

Early Over-nutrition HFL

Early Over-nutrition HFO

Group effect

Diet effect

Group × diet

n

7

7

9

6

5

6

   

# litters

3

3

5

3

3

6

   

Body weight (g)

46.9

52.3

50.9

50.3

55.1

54.1

7.10

5.58

0.05

(45.3–49.2)

(50.1–53.5)

(47.9–53.2)

(46.6–53.7)

(50.6–58.5)

(49.7–57.8)

§p = 0.012

†p = 0.008

p = 0.954

Glucose (mmol/L)

8.8 a

33.3 b

8.7 a

8.3 a

13.4 ab

26.4 b

F = 0.03

F = 9.52

F = 8.11

(8.3–10.1)

(8.6–33.3)

(7.8–13.5)

(7.1–9.0)

(10.2–22.7)

(12.2–29.2)

p = 0.8715

p < 0.001

*p = 0.001

Insulin (ng/mL)

1.29 a

0.68 a

4.94 b

1.32 a

1.60 a

1.04 a

F = 6.50

F = 6.39

F = 5.77

(1.02–2.72)

(0.43–2.65)

(3.40–7.40)

(0.64–1.59)

(0.47–2.97)

(0.78–2.18)

p = 0.016

p = 0.005

*p = 0.008

Leptin (ng/mL)

12.51 a

7.92 a

47.94 b

21.37 a

26.97 a

21.28 a

F = 0.54

F = 6.14

F = 10.84

(9.56–21.62)

(5.78–33.06)

(40.05–58.53)

(15.22–35.16)

(21.44–33.40)

(8.03–29.85)

P = 0.468

P = 0.006

*P < 0.001

EWAT (g)

0.926

1.016

0.986

1.033

1.347

1.066

F = 4.41

F = 4.25

F = 1.30

(0.816–0.980)

(0.962–1.215)

(0.964–1.154)

(0.938–1.174)

(1.086–1.660)

(0.740–1.376)

§p = 0.043

‡p = 0.023

p = 0.287

EWAT (g/100 g bw)

1.862

1.919

2.011

2.117

2.428

2.038

F = 1.90

F = 3.28

F = 2.11

(1.804–2.059)

(1.853–2.298)

(1.886–2.264)

(1.912–2.189)

(2.148–2.842)

(1.400–2.251)

p = 0.1770

p = 0.050

p = 0.136

mWAT (g)

0.667

0.715

1.008

0.982

1.558

1.160

F = 11.94

F = 1.70

F = 1.25

(0.609–0.825)

(0.625–0.940)

(0.889–1.140)

(0.848–1.147)

(0.905–1.672)

(0.993–1.404)

§p = 0.002

p = 0.197

p = 0.298

mWAT

1.394

1.419

2.042

2.007

2.618

2.214

F = 10.16

F = 0.73

F = 1.78

(g/100 g bw)

(1.347–1.646)

(1.229–1.757)

(1.810–2.157)

(1.668–2.341)

(1.787–2.970)

(1.746–2.419)

§p = 0.001

p = 0.491

p = 0.184

rpWAT (g)

0.217

0.235

0.206

0.248

0.247

0.296

F = 5.83

F = 0.28

F = 0.75

(0.167–0.279)

(0.182–0.252)

(0.155–0.229)

(0.228–0.291)

(0.214–0.322)

(0.234–0.351)

§p = 0.021

p = 0.759

p = 0.479

rpWAT (g/100 g bw)

0.451

0.459

0.384

0.502

0.448

0.559

F = 3.94

F = 0.20

F = 0.74

(0.369–0.566)

(0.371–0.477)

(0.330–0.453)

(0.458–0.543)

(0.410–0.563)

(0.437–0.619)

p = 0.0554

p = 0.819

p = 0.483

Liver (g)

2.018

2.849

2.297

2.465

2.787

3.245

F = 8.46

F = 6.62

F = 0.30

(1.934–2.142)

(2.550–2.890)

(2.210–3.036)

(2.173–2.762)

(2.541–3.623)

(2.540–3.582)

§p = 0.006

†p = 0.004

p = 0.744

Liver (g/100 g bw)

4.165

5.455

4.951

4.883

5.454

6.030

F = 4.55

F = 4.02

F = 0.32

(4.062–4.740)

(5.007–5.840)

(4.441–5.763)

(4.555–5.440)

(4.680–6.429)

(5.030–6.395)

§p = 0.040

‡p = 0.027

p = 0.727

  1. Values represent median (interquartile range). LF: 10 kcal% fat diet; HFL: 45 kcal% fat lard-based diet; HFO: 45 kcal% fat olive oil/fish oil-based diet; eWAT epididymal white adipose tissue; mWAT mesenteric white adipose tissue; rpWAT retroperitoneal white adipose tissue; bw body weight. Analysis: two-way ANOVA, Tukey’s correction for multiple comparisons.
  2. *Significant group × diet interaction effect, with differences (p < 0.05) denoted by differing letter superscripts per condition. Significant main effects indicated only in absence of interaction effect.
  3. §Main effect of group, early overnutrition > control.
  4. †Main effect of diet, HFL = HFO > LF.
  5. ‡Main effect of diet, HFL > HFO = LF. For leptin and insulin measures, n = 6 per group.
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