Figure 1
Schematic view of the multi-volume hemacytometer. (a) The counting chamber was attached to a glass slide that had an inlet for pipetting and an outlet for airflow. The cell counting chamber consisted of four chambers of varying depths including 0.1, 0.2, 0.4, 0.8 mm respectively. (b) The PDMS fabrication device. (c) Counting cell number in each chamber are proportional to the volume of the chamber when a sample flowed in the chambers having different volumes. In case of low cell concentration sample, slope which mean cells per unit volume is small. However, in high cell concentration sample, the slop is large due to large different of counting cells per chamber.
