Table 1 Comparison of cell concentration measurements between a standard hemacytometer and a multi-volume hemacytometer.

1

10⁶ cells/ml

1.08 ± 0.11 (9.7%)

1.14 ± 0.02 (1.6%)

0.99 ± 0.07 (7.0%)

1.14 ± 0.07 (5.7%)

1.02 ± 0.03 (3.3%)

1.04 ± 0.03 (2.5%)

2

1.18 ± 0.13 (10.8%)

1.14 ± 0.11 (9.3%)

0.94 ± 0.06 (6.7%)

1.08 ± 0.10 (9.6%)

1.14 ± 0.05 (4.5%)

1.12 ± 0.04 (3.6%)

3

1.05 ± 0.09 (8.5%)

1.07 ± 0.16 (14.9%)

1.09 ± 0.15 (17%)

1.08 ± 0.16 (14.4%)

1.07 ± 0.01 (1.3%)

1.07 ± 0.03 (3.1%)

4

1.16 ± 0.12 (10.5%)

0.88 ± 0.06 (6.5%)

0.94 ± 0.04 (4.6%)

1.08 ± 0.15 (14.0%)

1.12 ± 0.07 (6.5%)

1.10 ± 0.03 (3.0%)

5

1.19 ± 0.14 (11.3%)

1.12 ± 0.17 (15.2%)

0.92 ± 0.06 (6.8%)

0.94 ± 0.07 (7.2%)

1.05 ± 0.13 (13.3%)

1.03 ± 0.11 (10.4%)

AVG

1.13 ± 0.06

1.07 ± 0.11

0.98 ± 0.08

1.07 ± 0.08

1.08 ± 0.06

1.07 ± 0.04

%RSD

5.61

10.51

6.89

6.86

4.54

3.64

1

10⁵ cells/ml

1.38 ± 0.35 (25.3%)

1.26 ± 0.27 (21.4%)

1.14 ± 0.20 (17.4%)

0.94 ± 0.12 (11.5%)

1.22 ± 0.10 (8.3%)

1.17 ± 0.02 (1.5%)

2

1.24 ± 0.42 (34.0%)

1.12 ± 0.34 (30.5%)

1.07 ± 0.15 (14.3%)

1.18 ± 0.30 (25.7%)

1.26 ± 0.08 (6.4%)

1.23 ± 0.09 (7.7%)

3

1.20 ± 0.43 (35.8%)

1.16 ± 0.49 (42.1%)

1.28 ± 0.47 (36.9%)

1.20 ± 0.27 (22.3%)

1.21 ± 0.11 (9.0%)

1.21 ± 0.11 (9.4%)

4

1.22 ± 0.35 (28.6%)

1.16 ± 0.43 (37.4%)

1.21 ± 0.19 (15.3%)

1.28 ± 0.29 (22.5%)

1.26 ± 0.12 (9.6%)

1.26 ± 0.13 (10.2%)

5

1.20 ± 0.53 (44.1%)

1.00 ± 0.16 (15.8%)

1.28 ± 0.27 (21.1%)

1.13 ± 0.23 (19.9%)

1.30 ± 0.10 (8.0%)

1.26 ± 0.10 (8.1%)

AVG

1.25 ± 0.08

1.14 ± 0.09

1.20 ± 0.09

1.15 ± 0.13

1.25 ± 0.04

1.23 ± 0.04

%RSD

6.06

8.23

7.63

11.10

6.06

3.17

1

10⁴ cells/ml

1.40 ± 1.14 (81.4%)

1.40 ± 1.14 (81.4%)

1.20 ± 0.57 (47.5%)

1.15 ± 0.14 (11.9%)

1.18 ± 0.14 (12.1%)

1.17 ± 0.10 (8.7%)

2

1.00 ± 0.71 (70.7%)

1.00 ± 1.00 (100%)

1.10 ± 0.65 (59.3%)

1.15 ± 0.42 (36.4%)

1.20 ± 0.19 (15.8%)

1.22 ± 0.12 (9.5%)

3

0.60 ± 0.55 (91.3%)

1.40 ± 0.89 (63.9%)

1.20 ± 0.57 (47.5%)

1.15 ± 0.38 (33.0%)

1.15 ± 0.27 (23.6%)

1.16 ± 0.22 (19.0%)

4

1.00 ± 0.71 (70.7%)

1.20 ± 0.83 (69.7%)

1.20 ± 0.57 (47.5%)

1.10 ± 0.29 (25.9%)

1.28 ± 0.29 (22.4%)

1.24 ± 0.18 (14.3%)

5

1.00 ± 0.71 (70.7%)

0.80 ± 0.45 (55.9%)

1.00 ± 0.50 (50.0%)

1.15 ± 0.52 (45.1%)

1.18 ± 0.59 (22.1%)

1.16 ± 0.23 (19.9%)

AVG

1.00 ± 0.28

1.16 ± 0.26

1.14 ± 0.09

1.14 ± 0.02

1.20 ± 0.05

1.19 ± 0.04

%RSD

28.28

22.48

7.85

1.96

4.02

3.19

1

5 × 10³

cells/ml

4.00 ± 5.58

(136.9%)

8.00 ± 4.47

(55.9%)

2.00 ± 2.74

(136.9%)

7.00 ± 3.26

(46.6%)

5.50 ± 1.43

(25.9%)

5.65 ± 1.27

(22.4%)

2

6.00 ± 5.48

(91.3%)

4.00 ± 8.94

(223.6%)

9.00 ± 9.62

(106.9%)

6.00 ± 2.85

(47.5%)

5.25 ± 2.85

(54.3%)

5.54 ± 3.06

(55.3%)

3

2.00 ± 4.47

(223.6%)

4.00 ± 8.94

(223.6%)

4.00 ± 4.18

(104.6%)

7.50 ± 2.50

(33.3%)

6.00 ± 1.63

(27.2%)

6.16 ± 1.17

(19.0%)

4

2.00 ± 4.47

(223.6%)

6.00 ± 8.94

(149.1%)

7.00 ± 8.37

(119.5%)

6.00 ± 1.37

(22.8%)

6.25 ± 1.77

(28.3%)

6.24 ± 1.67

(28.8%)

5

8.00 ± 8.37

(104.6%)

2.00 ± 4.47

(223.6%)

6.00 ± 2.24

(37.3%)

5.50 ± 2.09

(38.0%)

5.25 ± 2.05

(39.1%)

5.29 ± 1.90

(36.0%)

AVG

4.40 ± 2.61

4.80 ± 2.28

5.60 ± 2.70

6.40 ± 0.82

5.65 ± 0.45

5.78 ± 0.41

%RSD

59.27

47.51

48.25

12.84

8.04

7.06

1

10³ cells/ml

0

0

0

1.50 ± 1.37

(91.3%)

1.50 ± 0.56

(37.3%)

1.41 ± 0.33

(23.6%)

2

2.00 ± 4.47

(223.6%)

0

0

1.50 ± 1.37

(91.3%)

1.25 ± 0.88

(70.7%)

1.22 ± 0.85

(69.9%)

3

4.00 ± 5.48

(136.9%)

2.00 ± 4.47

(223.6%)

1.00 ± 2.24

(223.6%)

2.00 ± 1.77

(70.7%)

2.00 ± 0.68

(34.2%)

2.05 ± 0.83

(40.6%)

4

2.00 ± 4.47

(223.6%)

2.00 ± 4.47

(223.6%)

1.00 ± 2.24

(223.6%)

2.50 ± 3.26

(163%)

1.75 ± 1.43

(81.4%)

1.76 ± 0.70

(39.4%)

5

0

0

1.00 ± 2.24

(223.6%)

2.50 ± 3.06

(122%)

1.75 ± 2.43

(81.4%)

1.84 ± 0.89

(48.3%)

AVG

1.60 ± 1.67

0.80 ± 1.10

0.60 ± 0.55

2.00 ± 0.50

1.65 ± 0.29

1.66 ± 0.33

%RSD

104.58

136.93

91.28

25.00

17.28

20.10