Figure 1
Prevalence of MAIT cells and other CD3+ populations in COVID-19 patients compared to uninfected individuals. (A) Gating strategy for identifying CD161 + Vα7.2 + MAIT cells in PBMCs done on a healthy control. (B) MAIT cell frequency in CD3+ population of PBMCs isolated from the mild and severe groups as compared to the control group. (C) Percentage of CD4 and CD8 double positive (DP) and double negative (DN) T-cell subpopulations in CD3+ cells of COVID-19 patients compared to unaffected individuals. (D) Percentage of the lymphocyte population identified in PBMCs isolated from peripheral blood of uninfected control individuals compared to COVID-19 mildly affected and severely affected patients and the prevalence of CD3+ cells in the lymphocyte population. (E) Percentage of MAIT cells expressing CD69 and (F) Mean fluorescence intensity (MFI) of CD69 in MAIT cells of healthy controls and COVID-19 patients reflecting the activation state of these cells. The Mann–Whitney test was conducted to compare each patient group with the control group in (B,D,F), error bars indicate the standard error of the mean. For (C,E), multiple t-tests with Holm-Sidak’s correction for multiple comparisons were conducted. (G) Histogram representation of CD69 expression in one healthy control, one mildly affected and one severely affected COVID-19 patient.
