Figure 5
From: Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains
Confirmation of developmental abilities after IVF using frozen–thawed spermatozoa cryopreserved using the EQ method. (A) Two-cell stage embryos at 24 h after insemination. (B) Blastocysts after in vitro culture for 120 h. (C) A litter born by Cesarian section from the transfer of 2-cell stage embryos. (D) Pups (black hair color) survived to weaning and the figure shows their foster mother (arrow) and mother’s pups (arrowheads). (E) Newborn pups from the mating of pairs of mice derived from frozen–thawed spermatozoa.
