Figure 6
From: Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains
Visualization of plasma membrane integrity and the acrosomal status. (A) Representative images of fresh and frozen–thawed spermatozoa by standard and EQ methods after staining using propidium iodide (PI; red) and peanut agglutinin (PNA; green). (B) The percentages of spermatozoa stained by PI ( +) and PNA ( +). Significant difference indicated by different superscript letters. (a, b; c, d; e, f P < 0.01, A, B P < 0.05 by Tukey–Kramer test).
