Table 1 Fertilization rates of oocytes following IVF using spermatozoa frozen by the standard or EQ methods.
From: Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains
Strain of males | Method | Ca2+ concentration (mM) | No. of epididymides used | Total no. (%) of oocytes fertilized/inseminated | Mean % of fertilization (± SEM) |
|---|---|---|---|---|---|
B6N | Standard | 2 | 8 | 427/469 (91.0) | 91.4 (± 1.5) |
EQ | 2 | 7 | 209/401 (52.1) | 49.9 (± 7.0)** | |
5 | 7 | 176/307 (57.3) | 56.7 (± 4.9)** | ||
B6J | Standard | 2 | 10 | 583/754 (77.3) | 77.3 (± 1.4) |
EQ | 2 | 7 | 118/380 (31.1) | 30.8 (± 2.0)** | |
5 | 7 | 150/301 (49.3) | 50.9 (± 10.5)* |
