Table 3 Fertilization rates following IVF using B6J sperm frozen by the standard method and stored for 2.5 years and the EQ method and stored for 1–3 months.

From: Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains

Method

Storage term at

No. of epididymides used

Total no. (%) of oocytes fertilized/ inseminated

Mean % of fertilization (± SEM)

−80 °C

−196 °C

Standard

–

2.5 years

6

177/199 (89.0)

89.0 (± 3.8)

EQ

3 months

–

5

41/165 (24.8)

24.9 (± 4.5)**

EQ

1 month

2 months

5

47/171 (27.5)

28.6 (± 4.9)**

  1. **P < 0.01 vs standard cryopreservation method by Mann–Whitney nonparametric U test. SEM, standard error of the mean.
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