Table 4 Full-term development of embryos fertilized in vitro using spermatozoa frozen by the standard method and stored for 2.5 years or by the EQ method and stored for 1–3 months.

From: Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains

Method

Storage term at

No. (%) of females

No. (%) of embryos

−80 °C

−196 °C

Transferred

Pregnant

(%)

Transferred

Implanted

(%)

Developed to offspring

(%)

Standard

–

2.5 years

10

10

(100)

156

123

(79)

91

(58)

EQ

3 months

–

3

3

(100)

40

26

(65)

25

(63)

EQ

1 month

2 months

3

3

(100)

38

26

(68)

21

(55)

  1. There were no significant differences in the implantation or birth rates between the groups by Fisher’s exact test.
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