Figure 3 | Scientific Reports

Figure 3

From: An engineered tetra-valent antibody fully activates the Tie2 receptor with comparable potency to its natural ligand angiopoietin-1

Figure 3

Effect of ASP4021 and related antibodies on vascular permeability in vivo. (a) Pharmacokinetics analysis of ASP4021 in rats. The concentration of ASP4021 in rat plasma after subcutaneous administration was measured using an ELISA-based assay. Each value indicates the mean ± SD of 3 rats per group. (b) Inhibitory effect of 2-16A2 and TIE-1-Igγ1-WT on vascular permeability in a rat mustard oil-induced vascular permeability model following treatment with 0.3 mg/kg. The vertical axis indicates the amount of leaked Evans Blue dye. The graph indicates the mean ± SEM of 6 rats per group. Student’s t-test was employed for statistical analysis. ****p < 0.0001 vs the vehicle group. (c) Inhibitory effect of ASP4021 on vascular permeability in a rat mustard oil-induced vascular permeability model following treatment with 0.1 or 0.3 mg/kg. Each graph indicates the mean ± SEM of 6 rats per group. Dunnett’s multiple comparisons test was employed for statistical analysis. ****p < 0.0001 vs the vehicle group.

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