Figure 4
Differential gene expression of subtilisins and TXNPx of promastigotes and axenic amastigotes of L. (V.) braziliensis clinical isolates. Transcript levels were evaluated by qRT-PCR and the resulting relative quantification (fold-change) values of subtilisins (LbrM.13.0860, silver bars), (LbrM.28.2570, nickel bars) and TXNPx (LbrM.15.1080, iron bars) are presented. Actin and protein S8 were used as endogenous controls. The ∆∆Ct value of each gene was calculated pair-to-pair between promastigotes and axenic amastigotes, and the promastigote sample with lowest expression for each gene was set as reference sample. The dashed lines indicate RQ level1 of the reference samples: NR1 promastigote for subtilisins; NR2 promastigote for TXNPx. The graph presents the mean of two independent experiments performed in triplicate. *p < 0.05. The figure was generated using GraphPad Prism version 9.0.1.
