Figure 1 | Scientific Reports

Figure 1

From: Effective silencing of miR-126 after ischemic stroke by means of intravenous α-tocopherol–conjugated heteroduplex oligonucleotide in mice

Figure 1The alternative text for this image may have been generated using AI.

Selection of the most effective antimiR sequence for targeting microRNA-126 (miR-126). (a) Schematic representation of the candidate antimiRs targeting miR-126. Blue, seed sequence; red, locked nucleic acids (LNAs); gray, DNAs (b, c) Efficiency of the candidate antimiRs measured by luciferase reporter assay. (b) The indicated antimiRs at 100 nM or (c) antimiR #7 at 3, 10, 30, and 100 nM were co-transfected with psiCHECK-2 reporter plasmid into bEnd3 cells. Relative luciferase activity was measured at 24 h after transfection. Abbreviations: miR-126 reporter, psiCHECK-2 reporter plasmid that contains the binding site for miR-126 in the 3′ UTR of the Renilla luciferase gene; psi, psiCHECK-2 empty vector that does not contain the binding site for miR-126. Data are presented as the mean ± s.e.m. (n = 3 per group).

Back to article page