Figure 2 | Scientific Reports

Figure 2

From: Effective silencing of miR-126 after ischemic stroke by means of intravenous α-tocopherol–conjugated heteroduplex oligonucleotide in mice

Figure 2The alternative text for this image may have been generated using AI.

Inhibition of microRNA in BECs after intravenous administration of Toc-HDO-antimiR #7. (a) Design of Toc-HDO-antimiR #7 and antimiR #7. (b) Quantitative reverse transcription PCR (qRT-PCR) analysis of miR-126 expression in the cerebral cortex from normal mice after intravenous administration of phosphate buffered saline (PBS), antimiR #7, or Toc-HDO-antimiR #7 at 5 mg/kg or 50 mg/kg (*P < 0.05, **P < 0.01 versus PBS control; ††P < 0.01 versus antimiR #7). (c) Time course of miR-126 expression in the ischemic region after permanent middle cerebral artery occlusion (pMCAO) (**P < 0.01 versus sham-operated mice). (d) qRT-PCR analysis of miR-126 expression in the cerebral cortex from sham-operated mice and the ischemic region from pMCAO model mice after intravenous administration of PBS, antimiR #7, or Toc-HDO-antimiR #7 at 5 mg/kg or 50 mg/kg (**P < 0.01 versus PBS control; †P < 0.01 versus antimiR #7). All data are relative to U6 small RNA levels and are presented as the mean ± s.e.m (n = 3 per group). P values were calculated using one-way ANOVA followed by (c) Dunnett’s test or (b, d) Tukey–Kramer test.

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