Figure 4 | Scientific Reports

Figure 4

From: Effective silencing of miR-126 after ischemic stroke by means of intravenous α-tocopherol–conjugated heteroduplex oligonucleotide in mice

Figure 4The alternative text for this image may have been generated using AI.

Upregulation of the miR-126 downstream genes by Toc-HDO-antimiR #7 in vitro under ischemia. (a) qRT-PCR analysis of the level of miR-126 and the mRNA levels of the candidate miR-126 downstream genes, Vcam1, Vegfa, Akt2, and Sgpl1, in bEnd3 cells transfected with antimiR #7 at 3 nM or 30 nM. (b) qRT-PCR analysis of the post- oxygen–glucose deprivation (OGD) level of miR-126 and mRNA levels of the miR-126 downstream genes in bEnd3 cells transfected with Toc-HDO-antimiR #7 at 3 nM or 30 nM. Expression levels are relative to U6 small RNA levels for miR-126 expression experiments and glyceraldehyde-3-phosphate dehydrogenase (Gapdh) mRNA levels for mRNA experiments. Data are presented as the mean ± s.e.m. (n = 3 per group; *P < 0.05, **P < 0.01 versus non-treated control). P values were calculated using one-way ANOVA followed by Dunnett’s test.

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