Table 3 Nucleotide sequences of primer pairs applied for the real-time PCRs, the product length and the temperature of anellation.
From: Immunomodulatory role of Parkinson’s disease 7 in inflammatory bowel disease
Gene | Primer pairs | Product length (bp) | Ta (°C) |
|---|---|---|---|
hPARK7 | Forward: 5′-AGT ACA GTG TAG CCG TGA-3′ Reverse: 5′-TAA TCT GGG CGC ACA GAA TT-3′ | 116 | 60 |
hTNFA | Forward: 5′-GAG GCG CTC CCC AAG AAG ACA-3′ Reverse: 5′-TGG GCC AGA GGG CTG ATT AGA-3′ | 182 | 60 |
hTGFB1 | Forward: 5′-GCG TGC GG CAG CTG TAC ATT GAC T-3′ Reverse: 5′-CGA GGC GCC CGG GTT ATG C-3′ | 174 | 60 |
hIL1B | Forward: 5′-CAC GCT CCG GGA CTC ACA G -3′ Reverse: 5′-GCC CAA GGC CAC AGG TAT TTT-3′ | 160 | 56 |
hIL6 | Forward: 5′-AAA GAT GGC TGA AAA AGA TGG AT-3′ Reverse: 5′-CTC TGG CTT GTT CCT CAC TAC TCT-3′ | 146 | 60 |
hIL10 | Forward: 5′-ATG CCC CAA GCT GAG AAC CAA GAC-3′ Reverse: 5′-AGA AAT CGA TGA CAG CGC CGT AGC-3′ | 107 | 60 |
hGAPDH | Forward: 5′-CAC CAC CAT GGA GAA GGC TG-3′ Reverse: 5′-GTG ATG GCATGG ACT GTG-3′ | 240 | 60 |
hRPLP0 | Forward: 5′-GAG GCT GCC AAC CGG AAC AAT GAC-3′ Reverse: 5′-TCC TGC AGG CGG CCA ATA GTG TCT-3′ | 206 | 60 |
mPark7 | Forward: 5′-TTT ATC TGA GTC GCC TAT GGT G-3′ Reverse: 5′-TTT GGA TGC AAG GTC ACA AC-3′ | 132 | 60 |
mTnfa | Forward: 5′-GGG CCA CCA CGC TCT TCT GTC TA-3′ Reverse: 5′-GAG AGG GAG GCC ATT TGG GAA CTT-3′ | 83 | 56 |
mTgfb1 | Forward: 5′-GTG CGG CAG CTG TAC ATT GAC TTT-3′ Reverse: 5′-GGC TTG CGA CCC ACG TAG TAG AC-3′ | 239 | 59 |
mIl17 | Forward: 5′-AGG ACT TCC TCC AGA ATG T-3′ Reverse: 5′-CCG CAA TGA AGA CCC TGA-3′ | 136 | 60 |
mIl1b | Forward: 5′-GCC ACC TTT TGA CAG TGA TGA GAA-3′ Reverse: 5′-GAT GTG CTG CTG CGA GAT TTG A-3′ | 36 | 55 |
mIl6 | Forward: 5′-AAC CAC GGC CTT CCC TAC TTC A-3′ Reverse: 5′-TGC CAT TGC ACA ACT CTT TTC TCA-3′ | 155 | 55 |
mIl10 | Forward: 5′-CAA AGG ACC AGC TGG ACA ACA TAC-3′ Reverse: 5′-GCC TGG GGC ATC ACT TCT ACC-3′ | 124 | 54 |
mGapdh | Forward: 5′-ATC TGA CGT GCC GCC TGG AGA AAC-3′ Reverse: 5′-CCC GGC ATC GAA GGT GGA AGA GT-3′ | 164 | 60 |
mRn18s | Forward: 5′-AGC GGT CGG CGT CCC CCA ACT TCT-3′ Reverse: 5′-GCG CGT GCA GCC CCG GAC ATC TA-3′ | 107 | 60 |
