Figure 3

Temporal and spatial expression of linear LMP-2 and circLMP-2_e5. (a, b) Kinetic expression of EBV linear LMP-2 and circLMP-2_e5, as well as EBV lytic genes in (a) GM12878 and (b) P3HR1 cells were carried out for the indicated time period using RT-qPCR. Data was normalized to RPL32/UBC and represents the mean ± SD of two independent experiments. (c) RT-qPCR analysis using junction-specific divergent primers showed LMP-2 and circLMP-2_e5 were located in both nucleus and cytoplasm of GM12878 cells. Data was normalized to the RNA yield ratio and represents the mean ± SD of two independent experiments.