Figure 3

Reproducibility of CL-O2−· and FL-OCl− measurements in blood. (A) Time courses of CL-O2−· (solid lines) and FL-OCl− (dotted lines) in blood. Four channels of CFL-H2400 were used simultaneously. The left vertical axis shows CL-O2−· intensity; the right vertical axis, FL-OCl− intensity. PMA was added at 150 s (arrow line). The baseline heights of FL-OCl− were adjusted at the same level. (B) Baseline-subtracted total amount of CL-O2−· and FL-OCl− calculated from the data in (A). Error ratios of the four measurements were 2.62% for the total amount of CL-O2−· and 1.74% for that of FL-OCl−.