Figure 3 | Scientific Reports

Figure 3

From: Embelin downregulated cFLIP in breast cancer cell lines facilitate anti-tumor effect of IL-1β-stimulated human umbilical cord mesenchymal stem cells

Figure 3

Embelin induced activation of caspase proteins in breast cancer cells. (a, b) MDA-MB-231 (c, d) MCF-7 and (e, f) MDA-MB-453 were treated with 25 and 50 μM embelin for 24 h. The cleaved caspase-8 expression and quantitative graphs of the Western blot results. (g, h) MDA-MB-231 (i, j) MCF-7 and (k, l) MDA-MB-453 were treated with 25 and 50 μM embelin for 24 h. The cleaved caspase-3 expression and quantitative graphs of the Western blot results. (m, n) MDA-MB-231 (o, p) MCF-7 and (q, r) MDA-MB-453 were treated with 25 and 50 μM embelin for 24 h. The cleaved caspase-9 expression and quantitative graphs of the Western blot results. The gray analysis of Western blots was normalized with β-actin. The full-length Western blots were shown in Supplementary Fig. S3. Data were shown as mean ± SEM and analyzed by one-way ANOVA with Dunnett's test. (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001).

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