Figure 4

The majority of CD11b⁺Ly6C^lo cells that develop in the liver after IRE treatment are side-scatter high, and express high levels of F4/80 and intermediate levels of MHCII. (a) Representative flow cytometry density plots showing Ly6C expression versus side-scatter (SSC), F4/80, MHCII, and CD64 of CD11b⁺ monocytes/macrophages isolated from the IRE-treated liver lobe compared to parts of the liver not directly treated by IRE (control) from the same animal. Rectangular gates define Ly6C^lo cells that are “high” for the given marker. (b) Quantification of CD11b⁺Ly6C^lo cells that are SSC, F4/80, MHCII, or CD64 high, as defined by the gates in (a), in control and IRE-treated parts of the liver. Two-tailed unpaired t tests show ***p < 0.001 and ****p < 0.0001. Individual points represent data from separate animals (n = 4). Graph bars and error bars show mean ± SEM. (c) Representative flow cytometry density plots showing that the majority (70%) of CD11b⁺Ly6C^lo cells in IRE-treated liver are SSC^hi, and that this subset is also F4/80^hi and MHCII^int. The remainder of the CD11b⁺Ly6C^lo cells that are SSC^lo (30%) express lower levels of F4/80 and very high levels of MHCII.