Table 1 Mean of experimental and predicted values of simultaneous biosorption of Congo red and cobalt by E. intestinalis using FCCCD matrix.

From: Bioprocess development for biosorption of cobalt ions and Congo red from aquatic mixture using Enteromorpha intestinalis biomass as sustainable biosorbent

Std	Run	Type	X₁	X₂	X₃	X₄	X₅	Congo red adsorption (%)			Cobalt adsorption (%)
Std	Run	Type	X₁	X₂	X₃	X₄	X₅	Actual	Predicted	Residuals	Actual	Predicted	Residuals
17	1	Fact	− 1	− 1	− 1	− 1	1	46.01	47.35	− 1.34	20.5	19.38	1.12
38	2	Axial	0	0	1	0	0	34.00	37.75	− 3.76	39.2	40.34	− 1.14
5	3	Fact	− 1	− 1	1	− 1	− 1	48.71	46.25	2.47	20.73	20.26	0.47
25	4	Fact	− 1	− 1	− 1	1	1	48.53	47.75	0.79	73.2	73.24	− 0.04
36	5	Axial	0	1	0	0	0	48.40	45.14	3.25	60.05	59.39	0.66
9	6	Fact	− 1	− 1	− 1	1	− 1	52.29	53.28	− 0.98	65.14	66.73	− 1.59
6	7	Fact	1	− 1	1	− 1	− 1	23.76	24.56	− 0.80	40.29	39.12	1.17
2	8	Fact	1	− 1	− 1	− 1	− 1	38.73	40.00	− 1.28	45.89	46.61	− 0.72
27	9	Fact	− 1	1	− 1	1	1	40.94	40.95	− 0.01	78.18	78.15	0.03
26	10	Fact	1	− 1	− 1	1	1	98.20	96.81	1.39	80.1	78.67	1.43
40	11	Axial	0	0	0	1	0	52.84	53.22	− 0.38	62.14	63.11	− 0.97
15	12	Fact	− 1	1	1	1	− 1	29.30	28.07	1.24	53.5	54.00	− 0.50
16	13	Fact	1	1	1	1	− 1	29.23	28.90	0.33	46.53	45.94	0.59
34	14	Axial	1	0	0	0	0	46.17	47.42	− 1.25	34.7	33.63	1.07
43	15	Center	0	0	0	0	0	41.00	47.02	− 6.02	49.13	48.71	0.42
23	16	Fact	− 1	1	1	− 1	1	63.14	61.51	1.63	26.57	25.23	1.34
8	17	Fact	1	1	1	− 1	− 1	6.19	5.84	0.35	17.34	18.54	− 1.20
3	18	Fact	− 1	1	− 1	− 1	− 1	52.19	53.24	− 1.05	22.9	22.33	0.57
39	19	Axial	0	0	0	− 1	0	40.34	41.50	− 1.16	23.4	22.48	0.92
32	20	Fact	1	1	1	1	1	25.94	27.37	− 1.43	46.32	46.51	− 0.19
29	21	Fact	− 1	− 1	1	1	1	37.91	38.30	− 0.39	69.43	68.92	0.51
13	22	Fact	− 1	− 1	1	1	− 1	38.13	37.76	0.37	55.37	53.90	1.47
48	23	Center	0	0	0	0	0	50.37	47.02	3.35	48.99	48.71	0.28
44	24	Center	0	0	0	0	0	47.18	47.02	0.16	49.5	48.71	0.79
20	25	Fact	1	1	− 1	− 1	1	30.70	33.26	− 2.56	17.2	18.10	− 0.90
37	26	Axial	0	0	− 1	0	0	55.87	53.66	2.21	50.37	49.29	1.08
30	27	Fact	1	− 1	1	1	1	67.24	65.90	1.34	65.25	65.55	− 0.30
7	28	Fact	− 1	1	1	− 1	− 1	48.71	52.34	− 3.62	16.3	17.55	− 1.25
47	29	Center	0	0	0	0	0	47.18	47.02	0.16	47.9	48.71	− 0.81
35	30	Axial	0	− 1	0	0	0	53.10	57.90	− 4.80	66.5	67.22	− 0.72
24	31	Fact	1	1	1	− 1	1	18.19	16.97	1.22	14.5	13.04	1.46
18	32	Fact	1	− 1	− 1	− 1	1	50.35	49.09	1.26	33.74	33.87	− 0.13
50	33	Center	0	0	0	0	0	48.86	47.02	1.83	46.8	48.71	− 1.91
49	34	Center	0	0	0	0	0	52.30	47.02	5.28	57.42	58.12	− 0.70
42	35	Axial	0	0	0	0	1	41.14	44.88	− 3.74	85.22	85.35	− 0.13
10	36	Fact	1	− 1	− 1	1	− 1	100.00	100.39	− 0.39	73.29	73.66	− 0.37
12	37	Fact	1	1	− 1	1	− 1	72.09	72.81	− 0.72	58.27	57.62	0.65
41	38	Axial	0	0	0	0	− 1	44.28	42.09	2.20	32.43	32.12	0.31
4	39	Fact	1	1	− 1	− 1	− 1	29.79	28.21	1.58	20.37	21.49	− 1.12
19	40	Fact	− 1	1	− 1	− 1	1	57.35	56.34	1.01	74.1	72.92	1.18
11	41	Fact	− 1	1	− 1	1	− 1	49.30	50.51	− 1.21	46.97	48.71	− 1.74
45	42	Center	0	0	0	0	0	47.67	47.02	0.64	63.2	63.72	− 0.52
14	43	Fact	1	− 1	1	1	− 1	63.47	63.40	0.07	18.82	18.95	− 0.13
1	44	Fact	− 1	− 1	− 1	− 1	− 1	41.68	40.23	1.45	33.9	34.89	− 0.99
22	45	Fact	1	− 1	1	− 1	1	40.76	39.72	1.04	29.3	30.42	− 1.12
33	46	Axial	− 1	0	0	0	0	45.85	46.14	− 0.29	28.3	29.20	− 0.90
21	47	Fact	− 1	− 1	1	− 1	1	59.24	59.45	− 0.21	67.71	67.74	− 0.03
31	48	Fact	− 1	1	1	1	1	24.73	24.58	0.15	65.14	65.72	− 0.58
28	49	Fact	1	1	− 1	1	1	65.04	65.20	− 0.16	50.5	48.71	1.79
46	50	Center	0	0	0	0	0	47.81	47.02	0.78	50.1	48.71	1.39

Variable	Variable code	Coded and actual levels
Variable	Variable code	− 1	0	1
Initial pH level	X₁	4	7	10
Algal biomass (g/L)	X₂	1	3	5
Congo red concentration (mg/L)	X₃	100	150	200
Cobalt concentration (mg/L)	X₄	40	120	200
Incubation time (min)	X₅	20	40	60

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Table 1 Mean of experimental and predicted values of simultaneous biosorption of Congo red and cobalt by E. intestinalis using FCCCD matrix.

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