Table 3 Analysis of variance for simultaneous biosorption of cobalt by E. intestinalis achieved by the FCCCD.
Source of variance | Degrees of freedom | Sum of square | Mean of square | F-value | P-value | Coefficient estimate | |
|---|---|---|---|---|---|---|---|
Overall model | 20 | 18,654.32 | 932.72 | 583.40 | < 0.0001* | 48.71 | |
Linear effect | X1 | 1 | 87.75 | 87.75 | 54.88 | < 0.0001* | 1.61 |
X2 | 1 | 521.44 | 521.44 | 326.15 | < 0.0001* | − 3.92 | |
X3 | 1 | 680.87 | 680.87 | 425.87 | < 0.0001* | − 4.48 | |
X4 | 1 | 14,028.93 | 14,028.93 | 8774.88 | < 0.0001* | 20.31 | |
X5 | 1 | 2.13 | 2.13 | 1.33 | 0.2578 | 0.25 | |
Interaction effect | X1X2 | 1 | 638.85 | 638.85 | 399.59 | < 0.0001* | − 4.47 |
X1X3 | 1 | 154.79 | 154.79 | 96.82 | < 0.0001* | − 2.20 | |
X1X4 | 1 | 163.71 | 163.71 | 102.40 | < 0.0001* | − 2.26 | |
X1X5 | 1 | 347.42 | 347.42 | 217.31 | < 0.0001* | − 3.30 | |
X2X3 | 1 | 74.12 | 74.12 | 46.36 | < 0.0001* | − 1.52 | |
X2X4 | 1 | 15.71 | 15.71 | 9.83 | 0.0039* | 0.70 | |
X2X5 | 1 | 3.23 | 3.23 | 2.02 | 0.1661 | − 0.32 | |
X3X4 | 1 | 400.02 | 400.02 | 250.21 | < 0.0001* | − 3.54 | |
X3X5 | 1 | 144.84 | 144.84 | 90.60 | < 0.0001* | 2.13 | |
X4X5 | 1 | 73.81 | 73.81 | 46.17 | < 0.0001* | 1.52 | |
Square effect | X12 | 1 | 688.17 | 688.17 | 430.44 | < 0.0001* | − 16.68 |
X22 | 1 | 526.83 | 526.83 | 329.52 | < 0.0001* | 14.59 | |
X32 | 1 | 37.53 | 37.53 | 23.47 | < 0.0001* | − 3.90 | |
X42 | 1 | 86.40 | 86.40 | 54.04 | < 0.0001* | − 5.91 | |
X52 | 1 | 207.74 | 207.74 | 129.94 | < 0.0001* | 9.16 | |
Error effect | Lack of fit | 22 | 33.02 | 1.50 | 0.79 | 0.6901 | |
Pure error | 7 | 13.34 | 1.91 | ||||
R2 | 0.9975 | SD | 1.26 | ||||
Adj R2 | 0.9958 | Mean | 46.85 | ||||
Pred R2 | 0.9925 | C.V. % | 2.70 | ||||
Adeq Precision | 88.24 | PRESS | 140.65 | ||||