Figure 2

MiR-513b-5p directly targeted COL1A1 and COL1A2 and inhibited their expression. (A) Schematic representation of miR-513b-5p binding sites in COL1A1-3′UTR and COL1A2-3′UTR. (B) The dual luciferase report assay confirmed the targeting relationship between miR-513b-5p and COL1A1-3′UTR. (C) The targeting relationship between miR-513b-5p and COL1A2-3′UTR was proved by the dual luciferase report assay. (D) The miR-513b-5p mimic and inhibitor transfected with the HASMCs. (E) MiR-513b-5p inhibited the COL1A1 mRNA expression. (F) MiR-513b-5p inhibited the COL1A2 mRNA expression. All data presented as mean ± standard deviation, and three independent experiments were carried out. Compared with mimic NC or inhibitor NC group, *p < 0.05, **p < 0.01, and ***p < 0.001. Student's t test with two-sided was performed.