Figure 3
MiR-513b-5p regulated the viability of the HASMCs by the RIP1-RIP3-MLKL and MMP pathways. (A) MTT assay was used to analyse HASMC cell viability. (B) LDH release assay was used to detect cell death. (C) Flow cytometric analysis was performed to detect cell apoptosis. (D) Western blot was used to detect the expression of related proteins in the RIP1-RIP3-MLKL pathway. (E) Analyze the expression of related proteins in the MMP pathway. All data presented as the mean ± standard deviation, and three independent experiments were carried out. Compared with mimic NC or inhibitor NC group, *p < 0.05, **p < 0.01, and ***p < 0.001. Student's t test with two-sided was performed.
