Figure 4
COL1A1 rescued the effect of miR-513b-5p on viability of HASMCs by the RIP1-RIP3-MLKL and MMP pathways. (A) The protein and mRNA expression was detected after COL1A1 interference and over-expression. (B) Interference with COL1A1 enhanced the inhibitory effect of miR-513b-5p on the cell proliferation. (C) Over-expression of COL1A1 inhibited the inhibitory effect of miR-513b-5p on the cell proliferation. (D) Interference with COL1A1 enhanced the induction of cell death by miR-513b-5p. (E) Over-expression of COL1A1 inhibited the induction of cell death by miR-513b-5p. (F) Interference with COL1A1 enhanced the induction of cell apoptosis by miR-513b-5p. (G) Over-expression of COL1A1 inhibited the induction of cell apoptosis by miR-513b-5p. COL1A1 regulated the expression of related proteins in the (H) RIP1-RIP3-MLKL and (I) MMP pathways. All data presented as the mean ± standard deviation, and three independent experiments were carried out. Compared with mimic NC + siRNA NC group, *p < 0.05, **p < 0.01, and ***p < 0.001. Compared with miR-513b-5p mimic + siRNA NC group, #p < 0.05, ##p < 0.01, and ###p < 0.001. One-way ANOVA with two-sided LSD post hoc test was performed.
