Figure 3
Effect of CAP on the uptake of DOX or DOX-LIP by B16 cells. (a) Effect of CAP (60 s) on the uptake of DOX or DOX-LIP by B16 cells after 4 h of co-incubation of cells with 10 μM DOX (free or liposomal) (b). Uptake of DOX by cells after 4 h co-incubation (same conditions as those applied in a), based on FACS measurement’s (Mean Fluorescence Intensity). (c,d) Representative LSCM micrographs of B16 cells following incubation with DOX (10 µM) only, or after subjection of the cells to CAP (for 60 s). (e) FI values calculated from micrographs by Image J.
