Figure 4
Apoptosis effect in B16F10 melanoma cells after treatment with CAP and/or DOX. (a) Flow cytometry with annexin V and PI dyes on B16 cells subjected to CAP (15 s) and/or treated with 0.1 μM DOX (free or liposomal) for 48 h. Dot plots of four-quadrant analysis Q4 involves the unstained, Q1 involves the early apoptotic (Annexin V positive), Q2 involves the late-apoptotic (double positive) and Q3 involves the dead (PI positive) cells. The number in each corner quadrant presents the percentage of cells in a typical experiment (n = 3). (b) Mean numbers from all flow cytometry experiments. (c–f) Separate analysis for each cellular subset. * is used when a group is statistical significant compared to ctr/CAP group and # is used when two different columns are compared, as indicated in each specific graph.
