Figure 1

Effect of FLX on the number of MeCP2⁺ cells in the prefrontal cortex (PFC), M2, M1 and CA3. (A) Diagrams modified from the Franklin and Paxinos atlas²² (with permission) showing pre-defined areas of the mouse brain in which MeCP2⁺ cells were counted. Numbers beside the diagrams indicate the antero-posterior range of the atlas in which slices were collected. (B) Enlargements of the original pictures obtained by the VS120 microscope showing MeCP2⁺ cells (brown dots) in representative sections of the PFC, M1 and M2 motor cortices and CA3 of WT and Mecp2 HET (HET) mice treated with 10 mg/kg FLX or vehicle for 14 days. On day 15 mice were euthanised and their brain processed for MeCP2 immunohistochemistry. Scale bars, 100 µm. (C) Histograms, generated with the Prism software (version 7.02; URL link), show the mean number (± SEM) of MeCP2⁺ cells in the PFC, M1 and M2 and CA3 of WT and HET mice (6–14 mice/group). Black dots and squares indicate individual data points. Blue and red columns indicate treatment with vehicle and FLX, respectively. ***P < 0.001 versus WT-H2O; #P < 0.01 versus HET-H2O; §P < 0.002, §§P < 0.0001 versus WT-FLX (Sidak’s test).