Figure 2
From: Hepatic cell mobilization for protection against ischemic myocardial injury
Characterization of hepatic cells in the liver and ischemic myocardium. (A) Confirmation of eYFP-positive hepatic cells in the liver and ischemic myocardium of Alb-Cre/eYFP mice with 5-day MI-R injury by fluorescence and immunohistochemistry microscopy. Each pair of fluorescence and immunohistochemistry images was from the same specimen. Green: eYFP. Red: Antibody-labeled cardiac troponin I. Blue: Hoechst 33,258-stained cell nuclei. Brown: Antibody-labeled eYFP by immunohistochemistry. The length scale is for all panels. (B, C) Fluorescence micrographs showing eYFP-positive cells, CD45-positive cells, and cytokeratin 19 (CK19)-positive cells in the ischemic myocardium of an Alb-Cre/eYFP mouse with 5-day MI-R injury. Note co-localization of eYFP with CK19, suggesting the presence of bile ductular epithelial cells.
