Figure 3 | Scientific Reports

Figure 3

From: Elevated S-adenosylhomocysteine induces adipocyte dysfunction to promote alcohol-associated liver steatosis

Figure 3

DZA activates ATGL in 3T3-L1 adipocytes. Immunofluorescence images showing localization of (A) ATGL, (B) pATGL-Ser(406), (C) CGI-58 and (D) GOS2 in BODIPY 493/503 and DAPI stained control and DZA-treated 3T3-L1 adipocytes (Scale bar—20 µm) with a magnified images of a representative 100 µM DZA-treated cell showing localization of pATGL-Ser(406) and CGI-58 on the LD surface. (E) The fluorescence intensity of ATGL, (F) pATGL-Ser(406), (G) CGI-58 and (H) GOS2 expression in each group of cells normalized to % of control cells, (n = 4) experiments; confocal images of 31 random cells from CGI-58 mRNA expression in control and DZA-treated 3T3-L1 adipocytes, n = 5. Data are presented as the mean ± SEM; values not sharing a common letter significantly differ from each other at p ≤ 0.05.

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