Figure 8
DZA alters the expression of adipocyte differentiation markers. (A) Representative immunofluorescence image of (A) PPARγ and (B) C/EBPα in BODIPY 493/503 and DAPI stained control and DZA-treated 3T3-L1 adipocytes (Scale bar—20 µm) and fluorescence intensity of (C) PPARγ and (D) C/EBPα in representative DZA-treated cells normalized to % of control cells, n = 5 experiments; images of 32 random cells from each treatment group per experiment were captured using confocal microscope. (E) Relative mRNA expression levels of PPARγ in control and DZA-treated 3T3-L1 adipocytes (n = 6). Data are presented as the mean ± SEM; values not sharing a common letter significantly differ from each other at p ≤ 0.05.
