Table 5 Accuracy and precision of RT-qPCR amplification of SARS-CoV 2 RdRp and E genes from a lower respiratory tract mucus sample from the first patient confirmed to have COVID-19 in South Korea.

From: Validation of real-time RT-PCR for detection of SARS-CoV-2 in the early stages of the COVID-19 outbreak in the Republic of Korea

SARS-CoV-2 PFU

Inter CV%

F-value

P-value

Intra CV%

A. RdRP gene

1 × 105

2.47

0.98

0.36

0.64

1 × 104

2.49

0.11

0.75

1.02

1 × 103

2.56

0.61

0.46

0.75

1 × 102

2.71

0.21

0.66

1.21

1 × 101

2.68

0.00

0.99

0.26

1 × 100

1.88

0.35

0.57

0.74

B. E gene

1 × 105

3.47

0.01

0.93

0.43

1 × 104

2.10

0.01

0.91

0.39

1 × 103

2.42

0.00

0.95

0.16

1 × 102

1.55

0.00

0.98

0.20

1 × 101

1.39

0.00

1.00

0.74

1 × 100

0.71

1.36

0.29

1.02

  1. P value > 0.05 indicates no difference between days. F value < 5.99 indicates no difference between days. F(1,6; 0.05) = 5.99; CV(%): Coefficient of variation.
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