Figure 3 | Scientific Reports

Figure 3

From: Kinetic characterisation and inhibitor sensitivity of Candida albicans and Candida auris recombinant AOX expressed in a self-assembled proteoliposome system

Figure 3

Inhibitor dose response assays for AOX incorporated into proteoliposomes. (A–D) Dose–response assay with AF, CB, CD and SHAM against AOX proteins. The assay was performed in 200 µL 65 mM MOPS pH 7.5 with a starting concentration of 300 µM NADH. Points represent the mean value (darker shading) and individual replicates (lighter shading) of technical measurements and the continuous line is the best fit for a four-parameter sigmoid function. (E) IC50 values determined from the data presented in Panels A–D. All measurements are the average of 3 biological replications ± SD. For each inhibitor, C. albicans and C. auris IC50 values which are significantly different from TAO (i.e., within columns) are marked with asterisks (* p < 0.002 and ** p < 0.001). C. albicans and C. auris differ significantly from each other only for AF (p = 0.013).

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