Figure 5
From: Context-dependent effects of whole-genome duplication during mammary tumor recurrence
Tetraploid recurrent tumor cells demonstrate a competitive advantage in low serum culture. (A) Cell count of diploid and induced tetraploid cells over time. (B) The percentage of BrdU + diploid and tetraploid cells at different serum concentrations. (C) The percentage of diploid and tetraploid cells in S phase at different serum concentrations as measured by DNA content analysis and cell cycle modeling. (D) The percentage of diploid and tetraploid cells in G1 phase at different serum concentrations as measured by DNA content analysis and cell cycle modeling. (E) Colony formation assays of diploid and spontaneously formed tetraploid cells in different serum concentrations. (F) Quantification of colony number from (E). For (B–E), significance was determined using two-way ANOVA with Sidak’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001. (A–D and F) were generated using Graph Pad Prism 9 software (version 9.0.0 (86) for Mac, https://www.graphpad.com/scientific-software/prism/).
