Figure 7

Polyploid primary tumors exhibit increased chromosomal instability and evidence of an innate immune response. (A) Chromosome counts from metaphase spreads performed on early passage diploid and tetraploid primary tumors. Red bars represent median chromosome counts. Dashed lines represent diploid and tetraploid chromosome counts. (B) Volcano plot showing differentially expressed genes between diploid and tetraploid tumors. Fold-change is shown on x-axis and adjusted p-value as determined by DESeq2 is shown on y-axis. The dotted line indicates an adjusted p value of 0.05. (C) Selected GO Biological Process terms from the top 50 significantly enriched in tetraploid tumors (FDR < 0.05). (D) Selected gene sets enriched in tetraploid tumors by GSEA analysis (FDR q-value < 0.1). (E) Representative images of CD45 staining on diploid and WGD primary tumors. (F) Quantification of CD45 staining on diploid and WGD primary tumors. CD45 staining area is normalized to the total nuclear area. The mean and standard error of the mean are represented for each group. Significance was determined using Welch’s t-test. (A–D and F) were generated using Graph Pad Prism 9 software (version 9.0.0 (86) for Mac, https://www.graphpad.com/scientific-software/prism/).