Table 1 DNA and RNA oligonucleotide sequences.

From: An epigenetic GPI anchor defect impairs TLR4 signaling in the B cell transdifferentiation model for primary human monocytes BLaER1

Name

Sequence (shown 5′ to 3′)

qRT-PCR primers

TLR4 fwd

CCCTGAGGCATTTAGGCAGCTA

TLR4 rev

AGGTAGAGAGGTGGCTTAGGCT

MD-2 fwd

CTGAAGGGAGAGACTGTGAATAC

MD-2 rev

GGCTCCCAGAAATAGCTTCA

ITGAM fwd

ACAACCCTAACCCAAGATCAC

ITGAM rev

AAACAGCTCTCGTACCACTTT

FCGR1B fwd

CCTTGAGGTGTCATGCGTG

FCGR1B rev

AAGGCTTTGCCATTTCGATAGT

CD14 fwd

GATTACATAAACTGTCAGAGGC

CD14 rev

TCCATGGTCGATAAGTCTTC

PIGH fwd1

GCCATCTTCATCACCCTCTTAG

PIGH rev1

CTGAATGCCAAGGGAATCAATG

PIGH fwd2

GCTGGACTGCTTGATTGAAGTAT

PIGH rev2

AAGACAATGCTGGCCTTCTG

PIGH fwd3

AGTCATGGTGTCAAGCACATTA

PIGH rev3

TCCAATTTCTGTCTGGCCTTT

PIGH fwd4

GCCATTTACATGCAGAAGGT

PIGH rev4

GACTGTGTCCACCTGATGGT

PIGH fwd5

GAGCAGGTGACTGGAAATCTAA

PIGH rev5

CCGAAAGTGTTCTTTGCTGAC

PIGH fwd6

GCTAAGCATGTTCAGGTTTACTTT

PIGH rev6

CCAGTACTGAAGGGCTTGTT

PIGH fwd7

GGAGGATGAGCGGAGCTTT

PIGH rev7

AGCGAACGCAGCGAGAG

PIGH fwd8

GGCGTCATGGAGGATGAG

PIGH rev8

CAGAATTCCCGGCAGGAC

PIGH fwd9

GGTGATTTACTACCTCTGCATCTT

PIGH rev9

AAGACGGGTACTACTTGGGATA

PIGH fwd10

TTCCGTACTCCAAACCATCAG

PIGH rev10

ATTTCCAGTCACCTGCTCTATG

PIGH fwd11

GGGAGATGACTCTTAAGCCATAG

PIGH rev11

GCTCTAAGATGCACTACATCCATAA

PIGH fwd12

GCAGGTGACTGGAAATCTAAC

PIGH rev12

CTCCTTTGGTAAAGTAGGCAAA

PIGH gDNA fwd

TACTGCGTTCAGCTGATTTAGG

PIGH gDNA rev

CCTCCAGAGAGGCCTGTAATA

Cloning primers

PIGH GA fwd

GAATTTCGACCCGGATCCGCGGCCGCGCCACCATGGAGGATGAGC

PIGH/PIGA GA rev

CCCCTACCCGGTAGAATTCACGCGTTCACTTATCGTCGTCATCCTTGTAATC

PIGA GA fwd

GAATTTCGACCCGGATCCGCGGCCGCGCCACCATGGCCTGTAGAG

RNA stimuli

3pRNA, sense

ppp-GGCCGAGACCUCGAAGAGAACUCU

3pRNA, antisense

ppp-AGAGUUCUCUUCGAGGUCUCGGCC

IVT4 template for in vitro transcription, sense

TTGTAATACGACTCACTATAGGGACGCTGACCCAGAAGA

TCTACTAGAAATAGTAGATCTTCTGGGTCAGCGTCCC

IVT4 template for in vitro transcription, antisense

GGGACGCTGACCCAGAAGATCTACTATTTCTAGTAGATCT

TCTGGGTCAGCGTCCCTATAGTGAGTCGTATTACAA

9.2s

AGCUUAACCUGUCCUUCAA

DNA stimuli

G3-YSD, sense

GGGAAACTCCAGCAGGACCATTAGGG

G3-YSD, antisense

GGGTAATGGTCCTGCTGGAGTTTGGG

  1. The sequences of DNA and RNA oligonucleotides used for qRT-PCR, cloning and cell stimulation are shown.
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