Figure 7
Effects of HSV-1, CMV and their co-infection on the expression of MCP-1 and IL-8 in human trabecular meshwork (TM) cells. Primary TM cells from obtained ScienCell Research Labs were infected at a multiplicity of infection of 1 and observed at 12 h and 2 days post-infection (PI). Supernatants were collected and the levels of MCP-1 (A) and IL-8 (B) were determined by ELISA assay. Expression of CCR2 (C) and CXCR1 (D), which are the receptors of MCP-1 and IL-8, respectively, are compared in the Vero cells and human TM cells using real-time qPCR analyses. The mRNA expression levels of transforming growth factor (TGF)-β1 (E), TGF-β2 (F), α-SMA (G), and FN (fibronectin) (H) are shown. HSV-1 and CMV infection induced significantly increased expression of MCP-1 and IL-8 in TM cells, showing significant and robust increase of MCP-1 and IL-8 upon CMV infection at 12 h and 2 days PI. Also, the expressions of CCR2 and CXCR1, which are the receptors of MCP-1 and IL-8, respectively, were significantly increased in TM cells, relative to Vero cells, the cell line isolated from kidney epithelial cell. (***P < 0.001 vs. transcripts from the Vero cells using independent t test; *P < 0.05 and **P < 0.01 vs. transcripts from the mock infection within 12 h and 2 days PI using one way ANOVA with Dunnett’s multiple comparison, N = 3).
