Figure 4
Upper panel shows the dissociation rate constants (koff, in s−1) determined by SPR for the patients’ ADA; each point represents a single patient. Only some of these patients’ ADA were detectable by ELISA (blue), and these had significantly slower koff than the ADA not detectable by ELISA (red) (p < 0.001 Student’s T test). The koff value of the commercial anti-IFX antibody used for the calibration curve is shown for comparison (green). Lower panel shows the sensorgrams simulating the SPR binding signals of three different ADA, with identical concentration (1 × 10–8 M) and kon (1 × 105 M−1 s−1) but different koff, corresponding to the mean values shown in the upper panel.
