Figure 3 | Scientific Reports

Figure 3

From: Mechanisms that allow vaccination against an oncolytic vesicular stomatitis virus-encoded transgene to enhance safety without abrogating oncolysis

Figure 3The alternative text for this image may have been generated using AI.

A four-fold reduction in the number of transgene-specific CD8+ T cells significantly abrogated cytotoxicity. C57BL/6 mice were vaccinated intramuscularly with 1 × 108 IU of an adenovirus expressing human dopachrome tautomerase (hDCT). Ten days later, splenocytes were harvested and used for the negative magnetic selection of CD8+ T cells. These T cells were co-cultured with various ratios of B16-F10 melanoma cells that had been pre-treated for 24 h with recombinant interferon-gamma to upregulate the expression of major histocompatibility complex molecules to facilitate antigen presentation. In parallel, a second set of co-cultured cells were tested in which the number of effector cells were reduced four-fold. The cytotoxicity assay was run for 16 h. Means and standard errors are shown. The experiment was conducted once with eight replicates per group. Data were analyzed by two-way analysis of variance with Tukey’s multiple comparisons test.

Back to article page