Figure 4

Immunohistochemical localization of Aplnr (white), insulin (red), Glut 2 (green) and cell nuclei (DAPI, blue) in islets from pregnant mice at GD 12 exposed in early life to control (A) or LP (B) diet. Co-localization of Aplnr to Ins⁺ Glut2^LO cells is indicated by arrows. Bar represents 80 µm in (A) and 50 µm in (B). The percent Ins⁺ Glut2^LO Aplnr⁺ cells relative to all Ins⁺ cells is shown for total pancreas (C), extra-islet clusters (D) or within islets (E) for control (closed circles, black bars) or LP pregnancies (open circles, grey bars). Values represent mean ± SEM (n = 4–6) in non-pregnant females (NP) or at gestational day (GD) 9, 12 or 18. *p < 0.05, **p < 0.001 vs. control.