Figure 5
(A) Proliferation of INS1E cells measured by MTT assay in the presence or absence of Apelin (10 nM) or Apela (4 nM) with or without the addition of the Aplnr inhibitor ML221 (10 µM). Values represent mean ± SEM (n = 4–6). *p < 0.05 vs. control, **p < 0.01, ***p < 0.001 vs. Apelin or Apela alone. Cell proliferation of β-cells visualized by fluorescence immunohistochemistry for insulin (green) and Ki67 (red) in islets isolated from neonatal mice incubated without (B) or with Apelin (100 nM, C). Bar represents 50 µm. The percentage of Ki67-positive β-cells was quantified (D) in control culture and in the presence of 100 nM or 1 μM apelin. Values represent mean ± SEM (n = 5–6 replicates per experiment). *p < 0.05 vs. control.
