Figure 2
Morphological changes in human AM defects after trauma. In control AM specimens, we observed a layer of flattened AECs in the epithelial layer (A) and a dense region of collagen fibres in the fibroblast layer after four days of culture by IMF confocal microscopy and SHG imaging (B). After creation of a 0.8 mm defect in the AM specimen and culture for up to 4 days, we observed a population of highly contractile fibroblast-like cells that had crossed the defect site (C) with evidence of a greater intensity of the SHG signal close to the wound edge (WE) and polarization of collagen fibres (D). Blue signal is DAPI staining of nuclei detected by confocal imaging and red signals (collagen) were detected by SHG imaging. The dotted white lines show the border along the length of the WE in the AM specimen. Scale bar = 50 μM.
