Figure 7
Collagen fibre organization in AM defects after trauma. SHG imaging of collagen (red) showed dense collagen fibre alignment tangential to the wound edge (WE) in CAM and PAM specimens but not in control specimens (Top panel). After trauma, there was a region of highly polarized collagen fibres aligned in the direction at around 90’C in wounded specimens. This intense organization was absent in control specimens. Quantification of collagen fibres revealed a significant increase in the SHG intensity values for WE specimens than controls (Bottom panel). The dotted white lines show the length of the WE in the AM. Scale bar = 100 µm.
