Figure 6

TF & HM enrichment analysis function of AnnoMiner. The output of a TF & HM enrichment analysis in AnnoMiner is first a plot of the top 10 enriched transcription factors (a). The identity of the enriched TF with its combined score is shown. Hovering over the bar of a TF will reveal meta-information associated with the ChIP-seq dataset (b). Second and at the same time, a table is returned, which contains the information on all TFs stored in the AnnoMiner database and their enrichments in the submitted gene list. The table is sortable and downloadable. Once downloaded, it includes information on the sample, the target gene for each TF, as well as the score, FDR and combined score. Target gene lists can be copied to the clipboard for further analysis. For demonstrating the TF enrichment analysis function of AnnoMiner, we took advantage of a study identifying direct daf-16/FoxO targets from a longevity study (⁵³ and GEO dataset GSE72426). In this study, the authors looked for isoform-specific targets of daf-16. We combined the list of all DAF-16 isoform targets and submitted it to AnnoMiner for identifying enriched transcription factors. AnnoMiner displays the 10 most enriched TFs, with their combined score indicated in the plot (a) as well as the table of all TFs stored for C. elegans and including enrichment information for the submitted gene list. Daf-16 is the fourth hit; pqm-1, as well as elt-2, which both precede daf-16 in the hitlist, have been shown to bind to daf-16 response elements.